Ayogly et al. have designed an innovative suspension bead-array experimental approach for studying in one assay both antigen-specific antibody reactivity and complement activation induced C3 deposition.
The benefits of such a scalable approach is based on its capacity to measure multiplex antigen-specific complement activation in the context of an autoimmune disease (ex. Rheumatoid Arthritis) and viral antigen detection (ex. Epstein-Barr virus encoded Nuclear Antigen-1 (EBNA-1)).
The combination of antibody reactivity profiling and induced complement activation in one assay open new perspectives in autoantibody- and virus-induced immune diseases.
Want to know more?
Ayoglu B. et al. “Bead Arrays for Antibody and Complement Profiling Reveal Joint Contribution of Antibody Isotypes to C3 Deposition (5 May, 2014) PLoS ONE, CrossRef DOI Link to Publisher-Maintained copy: http://dx.doi.org/10.1371/journal.pone.0096403
Congratulations to the authors, who turned to tebu-bio’s range of products when selecting their antigens (ex. EBNA-1 protein) coupled to beads to determine the optimal assay conditions for measuring complement activation.