Both ROS (Reactive Oxygen Species) and RNS (Reactive Nitrogen Species) on the one hand, and the small G proteins belonging to the Rho family on the other hand, are key regulators in various signal transduction pathways.
More recently it has been suggested that crosstalk between reactive species and Rho GTPases plays a crucial role in some of their physiological functions. Furthermore, these crosstalk events have been linked to pathological processes, e.g. lung injury and cancer.
Our partner Cytoskeleton Inc. has summarized recent findings in the newsletter Rho GTPases and Reactive Oxygen Species: Crosstalk and Feedback. Let’s take a look at what’s available for research in this area.
State of the art small G protein activation measurements
To measure small G protein activation, in the past only pull-down experiments and kits were available. In these experiments, small G protein-specific binding domains coupled to beads are used. The final step during this experimental setup to estimate the activation status of the small G protein of interest, is a Western blot, which only allows for qualitative conclusions.
The innovative G-LISA technology developed by Cytoskeleton Inc., means researchers can now obtain quantitative data using much less volumes of sample. Furthermore, the results can be achieved much quicker, and in a set-up that’s as easy as an ELISA.
There’s a lot to learn about the G-LISA method here (technical info compiled by tebu-bio’s protein experts). This exciting technology is available for RhoA, Rac1, Cdc42, Ras, RalA, and Arf1 and 6.
By the way – a summer promotion with 10% discount is running until Mid July offering a good opportunity for researchers to try the method.
If you’re interested in exploring new solutions for your small G protein and oxidative stress related research projects, tebu-bio’s experts will be pleased to talk to you.