Measuring soluble collagen in biological samples is a straightforward experiment which can be done with the Sircol soluble collagen assay using Sirius Red dye (Sircol dye) in a convenient manner (we explored this method in a recent post). Nevertheless, up to now it has been a challenge to measure insoluble collagen.
During periods of rapid growth, development, tissue repair remodeling and wound healing, insoluble collagen fibers are laid down. Until now, an investigator seeking to measure covalent cross linked insoluble collagen required measurements based on free hydroxyproline content, a procedure that requires strong acid (6.0 M HCl), high temperatures (+ 950 C) and overnight cooking (18 to 24 hours).
Insoluble collagen measurements made easy
- Collagen soluble in cold dilute acid
- Collagen soluble in cold dilute acid and pepsin
- Insoluble collagen
Of course, by adding the results together a value for the “Total Collagen” in a sample can be established.
Interested in using Sircol assays or outsourcing them?